In- vitro Evaluation and Characterization of Prednisolone

Abstract

Prednisolone is a synthetic corticosteroid, used in many therapeutic actions like rheumatoid arthritis, asthma, allergy, pulmonary disease and other inflammatory and immunity diseases at any life stage. This study aimed to physical characterization of the prednisolone. these physical characterizations includes: determination of melting points by used two different methods: manual method by using capillary method and measuring by instrument by using the differential scanning calorimetry to detect a purity of active ingredient, Determination of λ max (maximum absorbance) of prednisolone in four different solvents (ethanol, DW, phosphate buffer pH 6.8 and 0.1 N HCl), Calibration curves of prednisolone at the same different solvents above, Flowability teste of the prednisolone powder by detecting of angle of repose, carr,s index and hausner ratio to determine its flowability and finally test the morphology of it by using Scanning Electron Microscopy at zooming 50 and 10 micrometer. The results from these physical characterizations are detect the purity of the prednisolone drug by measuring of the melting point of it, it has maximum absorbance at 242 nm in ethanol and 248 nm in other three solvents respectivly when scanned by using UV- spectroscopy, calibration curves obeyed Beer’s Lambert law within the concentrations used at four different types of solvent, very, very poor flowable of prednisolone after detected it by the flowability test and that’s mean the drug need to improve it and finally it have a crystal shape when detect it by scanning by Scanning Electron Microscopy.