Estimation of Prostate Specific Antigen (PSA) Concentrations in Patients with Prostatitis by Fully Automated ELISA Technique

Abstract

Only prostatic epithelial cells secrete prostate-specific antigen (PSA), therefore men with prostatic diseases, including cancer, have higher serum concentrations of PSA. We assessed its value in prostate cancer detection and staging. These studies aim to determine the PSA value and relation with body mass index Eighty patients with confirmed prostatitis were included in a case-control study and 60 persons as a control group. Three milliliters of venous blood were extracted using a disposable syringe in an aseptic manner from 80 patients and 60 healthy controls via venipuncture. After allowing the blood to coagulate in a gel tube, the serum was extracted using a 5-minute, 1500 rpm centrifugation. To be employed in the ELISA test, the serum was collected in an Eppendorf tube without ethylinditetracitic acid (EDTA) and then kept at ˉ20°C. The study found that the mean PSA level in the patient group was 7.65 ng/mL, whereas in the control group, it was 0.932 ng/mL. This difference was statistically significant (p = 0.001) , and significant relationship between PSA and BMI had an r-value of 0.207 (p = 0.18), also significant relationship between PSA and age showed an r-value of 0.178 (p = 0.16) These studies conclude that the body mass index consider as main factors for increase in prostatic specific antigen.