DNA Sequencing of Leishmania Sp. Isolated from Patient with Visceral Leishmaniasis in Wasit Governorate of Iraq

Abstract

Leishmania sp. is responsible for visceral leishmaniasis, a neglected and lethal parasitic disease. Leishmania strain obtained from Al-karama Hospital, in Wasit Governorate of Iraq, isolated from patient infected with visceral leishmaniasis. PCR technique was done for detection VL based on the kinetoplast DNA (kDNA). The current study also included DNA sequence analysis where the phylogenetic tree was constructed using Maximum Likelihood method in (MEGA. X version). The Leishmania donovani isolate kinetoplast minicircle DNA gene were showed closed genetic related to Leishmania donovani strain MHOM/SD/95/SIGIN kinetoplast minicircle DNA, complete sequence (AF169136.1) at total genetic changes (0.01%).The NCBI BLAST Homology identity (99%) of Leishmania donovani kinetoplast minicircle DNA, partial with NCBI Leishmania donovani strain MHOM/SD/95/SIGIN kinetoplast minicircle DNA (AF169136.1).