Virulence Factors of Multidrug Resistant Klebsiella Pneumoniae: a Comparative Genomic and Phenotypic Analysis

Abstract

Multidrug-resistant (MDR) Klebsiella spp. poses a significant challenge to hospitals worldwide. The aim of this study was to investigate the molecular and phenotypic characteristics of MDR Klebsiella pneumoniae and Klebsiella varicola isolates. Whole-genome sequencing (WGS) using in vitro assays was used to assess biofilm formation and in vivo pathogenicity assessment using a Galleria mellonella larval model

Our findings revealed a high prevalence of O1/O2 serotypes (67%), consistent with previous global data. Different K locus serotypes were identified, with ST15-KL19 being the most common (19%) and most closely associated with O1/O2v2 serotype Notably, a widespread Klebsiella varicola strain of O1/O2v2 serotype (ST10-KL151) was responsible the system went into bacterial genes . Significant differences in biofilm formation capacity were observed among species. One K. pneumoniae strain, ST280-KL23, showed more positive biofilms than the other nine isolates. In terms of infection, KL105-O1/O2v2 serotype exhibited the highest virulence, causing the most significant mortality in the Galleria mellonella sample

These data: MDR Klebsiellas pp. The study highlights the critical importance of continued molecular surveillance to identify key drug pathogens and alternative therapeutic strategies. Klebsiella parasites. There is a need to develop effective treatments for this multidrug-resistant pathogen and minimize public health impacts.