Study Antibacterial Activity of Pyocyanin Extracted from Pseudomonas Aeruginosa

Abstract

The study aimed to detect Pseudomonas aeruginosa pyocyanin genes and evaluate the
antibacterial activity of pyocyanin produced by P. aeruginosa isolated from burn infections. A total
of 224 specimens were collected from burn patients. Culture traits, biochemical testing, and Vitek-2
system confirmation were used to make the identification. P. aeruginosa was identified in 109
(48.6%) of bacterial isolates, 30 (27.5 %) produced pyocyanin, 45 (41.3 %) produced other
pigments and 34 (31.2 %) not produced any pigments. Genetically, study included used PCR
technique to investigation predominance of pyocyanin pigment production genes, exhibited higher.
Chloroform was used to extract pyocyanin from P. aeruginosa, and FTIR was used to identify it.
The extract of pyocyanin showed broad-spectrum antibacterial activity, with the greatest activity
against Gram negative. It had little effect on Staphylococcus aureus and P. fluorescens and but
shown significant antibacterial efficacy against Proteus mirabilis followed by Acinetobacter
baumanni, Escherichia coli and Klebsiella pneumonia.