To Study the Diagnostic Value of Isolation of Pure Microbial Culture and Methods of Isolation of Pure Culture

Abstract

In this article, detailed information on the methods of planting and replanting microorganisms in a dense and liquid nutrient medium was obtained and studied. First, we put 10 ml of sterile physiological solution in every 2-3 tubes 9 ml of GPA in 5-6 test tubes, graduated pipettes and 5-6 sterile Petri dishes, a mixture of several types of bacteria in a test tube (staphylococci, salmonella, hay bacillus). Cells of microorganisms grown in a liquid nutrient medium are taken in a sterile pipette, less often - with a needle. In order not to damage the cells of microorganisms, the hook (needle) is cooled by touching the inner surface of the container or the place where the microbe does not grow in the nutrient medium, and only then a smaller amount of microbial mass is taken. comparison of the results was studied.